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Suppression of Glutamate Carboxypeptidase II Ameliorates Neuronal Apoptosis from Ischemic Brain Injury.
- Source :
-
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association [J Stroke Cerebrovasc Dis] 2016 Jul; Vol. 25 (7), pp. 1599-1605. Date of Electronic Publication: 2016 Apr 05. - Publication Year :
- 2016
-
Abstract
- Background: Ischemia stroke is a destructive cerebrovascular disease and a major cause of death and lifelong neurological disability. N-Acetyl-l-aspartyl-l-glutamate (NAAG) is a neurotransmitter in the mammalian brain and involves a variety of physiological and pathological functions including ischemia brain injury. Full understanding of the functions of NAAG peptidase (GCPII) in the pathogenesis of ischemia brain injury is extremely valuable for effective therapies to ischemia stroke.<br />Methods: The expressions of GCPII and NAAG agonist metabotropic glutamate receptor (mGluR3) and TGFb1 were examined by real-time polymerase chain reaction and western blot. Moreover, GCPII knockdown cells were constructed using lentivirus-mediated transfection. Function and molecular mechanisms of GCPII knockdown on apoptosis induced from hypoxic-ischemic-induced injury in neuronal cells were analyzed.<br />Results: In this study, we found that the expressions of GCPII and mGluR3 were upregulated in CoCl2-induced hypoxia environment in neuronal cells. Moreover, knockdown of GCPII in neuronal cells ameliorated apoptosis from hypoxic-ischemic-induced injury through suppressing expressions of caspase 3 and caspase 9.<br />Conclusions: Our results highlighted the roles of GCPII in the ischemia brain injury, and might provide an important target in therapeutic implications.<br /> (Copyright © 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Brain pathology
Caspase 3 genetics
Caspase 3 metabolism
Caspase 9 genetics
Caspase 9 metabolism
Cell Hypoxia
Cell Line, Tumor
Cellular Microenvironment
Disease Models, Animal
Down-Regulation
Glutamate Carboxypeptidase II genetics
Infarction, Middle Cerebral Artery genetics
Infarction, Middle Cerebral Artery pathology
Neurons pathology
RNA Interference
RNA, Messenger genetics
RNA, Messenger metabolism
Rats, Sprague-Dawley
Receptors, Metabotropic Glutamate genetics
Receptors, Metabotropic Glutamate metabolism
Time Factors
Transfection
Transforming Growth Factor beta1 genetics
Transforming Growth Factor beta1 metabolism
Apoptosis
Brain enzymology
Glutamate Carboxypeptidase II metabolism
Infarction, Middle Cerebral Artery enzymology
Neurons enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-8511
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association
- Publication Type :
- Academic Journal
- Accession number :
- 27068862
- Full Text :
- https://doi.org/10.1016/j.jstrokecerebrovasdis.2015.10.035