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Molecular detection and extraction of pyrene in plasma and tissues of Sprague-Dawley rats.
- Source :
-
Cellular and molecular biology (Noisy-le-Grand, France) [Cell Mol Biol (Noisy-le-grand)] 2016 Mar 20; Vol. 62 (3), pp. 15-9. Date of Electronic Publication: 2016 Mar 20. - Publication Year :
- 2016
-
Abstract
- In this paper, an efficient method for determination of total pyrene concentration in the biological samples including plasma, liver, spleen, lung and kidney of Sprague-Dawley rats were investigated and established using steady-state fluorescence method. Equilibrium dialysis method was applied to determine plasma protein binding rate of pyrene. The results illustrated that the protein binding rate depends on the concentration of pyrene in plasma. Extraction of pyrene in plasma was studied by using biomedical nanopartical which was prepared from synthesized associating polymer poly(ethylene glycol) end-capped by hexadecane. The Critical Micelle Concentration (CMC) of the polymeric micelle in aqueous solution was determined to equal 0.0063 mg/mL using 1-pyrenemethanol as a fluorescent probe. The distribution of free pyrene and pyrene loaded nanoparticals in blood were determined. The results showed that over 95% of the free pyrene was distributed into the erythrocyte, and the pyrene-loaded nanoparticles were less distributed in to the erythrocyte than free pyrene, but it was higher than 60%. This study provides an efficient method to detect pyrene in different tissues as well as an extraction method at the molecular level, which might contribute to the development of modern molecular diagnosis and identification in vivo.
- Subjects :
- Alkanes chemistry
Animals
Drug Carriers chemistry
Erythrocytes metabolism
Fluorescent Dyes administration & dosage
Kidney metabolism
Liver metabolism
Male
Micelles
Nanotechnology
Pyrenes administration & dosage
Pyrenes blood
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Spleen metabolism
Fluorescent Dyes isolation & purification
Fluorescent Dyes pharmacokinetics
Nanoparticles chemistry
Polyethylene Glycols chemistry
Pyrenes isolation & purification
Pyrenes pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1165-158X
- Volume :
- 62
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cellular and molecular biology (Noisy-le-Grand, France)
- Publication Type :
- Academic Journal
- Accession number :
- 27064868