Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.

Authors :: Dias Kde C
Barbugli PA
Vergani CE
Source :: Journal of microbiological methods [J Microbiol Methods] 2016 Jun; Vol. 125, pp. 40-2. Date of Electronic Publication: 2016 Apr 07.
Publication Year :: 2016
Abstract: This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.<br /> (Copyright © 2016. Published by Elsevier B.V.)

Subjects :: Animals
Biofilms drug effects
Biofilms growth & development
Buffers
Cell Line
Cells, Cultured
HEPES chemistry
Humans
Hydrogen-Ion Concentration
Keratinocytes drug effects
Morpholines chemistry
Morpholines toxicity
Staphylococcus aureus drug effects
Cell Survival drug effects
Culture Media chemistry
HEPES pharmacology
Keratinocytes physiology
Morpholines pharmacology
Staphylococcus aureus growth & development

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