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A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.
- Source :
-
Anaerobe [Anaerobe] 2016 Jun; Vol. 39, pp. 114-6. Date of Electronic Publication: 2016 Apr 01. - Publication Year :
- 2016
-
Abstract
- We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Archaea metabolism
Biofuels analysis
Bioreactors
DNA Fingerprinting
DNA Restriction Enzymes genetics
DNA Restriction Enzymes metabolism
Deoxyribonucleases, Type II Site-Specific genetics
Deoxyribonucleases, Type II Site-Specific metabolism
Polymorphism, Restriction Fragment Length
Anaerobiosis genetics
Archaea classification
Archaea genetics
Databases, Genetic
Methane biosynthesis
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8274
- Volume :
- 39
- Database :
- MEDLINE
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 27046270
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2016.03.013