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The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains.
- Source :
-
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2016 Jun; Vol. 22 (6), pp. 574.e7-9. Date of Electronic Publication: 2016 Mar 31. - Publication Year :
- 2016
-
Abstract
- The French Reference Centre for chlamydiae uses two real-time PCRs targeting the pmpH gene of Chlamydia trachomatis to differentiate between L strains and variant L2b, responsible for a lymphogranuloma venereum outbreak in Europe. We compared the results obtained for 122 L2b C. trachomatis-positive specimens, using the two real-time PCRs, with the sequencing of the ompA gene. Only 91 specimens were confirmed as L2b. Our results demonstrate that the lymphogranuloma venereum outbreak is no longer dominated by the variant L2b, and that many L-positive specimens were misidentified as L2b with the method used, which raises the question of its specificity.<br /> (Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Disease Outbreaks
Europe epidemiology
Humans
Lymphogranuloma Venereum epidemiology
Male
Sensitivity and Specificity
Sequence Analysis, DNA
Bacterial Outer Membrane Proteins genetics
Chlamydia trachomatis isolation & purification
Diagnostic Errors
Lymphogranuloma Venereum diagnosis
Molecular Diagnostic Techniques methods
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1469-0691
- Volume :
- 22
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 27040805
- Full Text :
- https://doi.org/10.1016/j.cmi.2016.03.018