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MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker's yeast in lean dough.
- Source :
-
Microbial cell factories [Microb Cell Fact] 2016 Apr 04; Vol. 15, pp. 54. Date of Electronic Publication: 2016 Apr 04. - Publication Year :
- 2016
-
Abstract
- Background: Trehalose is related to several types of stress responses, especially freezing response in baker's yeast (Saccharomyces cerevisiae). It is desirable to manipulate trehalose-related genes to create yeast strains that better tolerate freezing-thaw stress with improved fermentation capacity, which are in high demand in the baking industry.<br />Results: The strain overexpressing MAL62 gene showed increased trehalose content and cell viability after prefermention-freezing and long-term frozen. Deletion of NTH1 in combination of MAL62 overexpression further strengthens freezing tolerance and improves the leavening ability after freezing-thaw stress.<br />Conclusions: The mutants of the industrial baker's yeast with enhanced freezing tolerance and leavening ability in lean dough were developed by genetic engineering. These strains had excellent potential industrial applications.
- Subjects :
- Cold Temperature
Flour microbiology
Gene Deletion
Gene Expression Regulation, Fungal
Organisms, Genetically Modified
Up-Regulation genetics
Acclimatization genetics
Fermentation genetics
Freezing
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Trehalase genetics
alpha-Glucosidases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2859
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- Microbial cell factories
- Publication Type :
- Academic Journal
- Accession number :
- 27039899
- Full Text :
- https://doi.org/10.1186/s12934-016-0453-3