Liquid Growth of Arrayed Fluorescently Tagged Saccharomyces cerevisiae Strains for Live-Cell High-Throughput Microscopy Screens.

Authors :: Cox MJ
Chong YT
Boone C
Andrews B
Source :: Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2016 Apr 01; Vol. 2016 (4), pp. pdb.prot088799. Date of Electronic Publication: 2016 Apr 01.
Publication Year :: 2016
Abstract: This protocol describes culturing arrays of fluorescently tagged yeast strains to early log-phase in a 96-well format for imaging on a high-throughput (HTP) microscope. The method assumes the use of the synthetic genetic array (SGA) technique to create the array of marked strains. When this approach is coupled with automated image analysis, the subcellular distribution and abundance of tagged proteins can be systematically and quantitatively examined in different genetic backgrounds and/or under different growth regimes.<br /> (© 2016 Cold Spring Harbor Laboratory Press.)

Subjects :: Automation, Laboratory
Image Processing, Computer-Assisted methods
Optical Imaging methods
Staining and Labeling methods
High-Throughput Screening Assays
Microscopy, Fluorescence methods
Saccharomyces cerevisiae growth & development

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