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In vitro and in vivo reduction of post-prandial blood glucose levels by ethyl alcohol and water Zingiber mioga extracts through the inhibition of carbohydrate hydrolyzing enzymes.
- Source :
-
BMC complementary and alternative medicine [BMC Complement Altern Med] 2016 Mar 31; Vol. 16, pp. 111. Date of Electronic Publication: 2016 Mar 31. - Publication Year :
- 2016
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Abstract
- Background: Type 2 diabetes is a serious problem for developed and developing countries. Prevention of prediabetes progression to type 2 diabetes with the use of natural products appears to be a cost-effective solution. Zingiber mioga has been used as a traditional food in Asia. Recent research has reported the potential health benefits of Zingiber mioga, but the blood glucose reducing effect has not been yet evaluated.<br />Methods: In this study Zingiber mioga extracts (water and ethanol) were investigated for their anti-hyperglycemic and antioxidant potential using both in vitro and animal models. The in vitro study evaluated the total phenolic content, the oxygen radical absorbance capacity (ORAC) and the inhibitory effect against carbohydrate hydrolyzing enzymes (porcine pancreatic α-amylase and rat intestinal sucrase and maltase) of both Zingiber mioga extracts. Also, the extracts were evaluated for their in vivo post-prandial blood glucose reducing effect using SD rat and db/db mice models.<br />Results: Our findings suggest that the ethanol extract of Zingiber mioga (ZME) exhibited the higher sucrase and maltase inhibitory activity (IC50, 3.50 and 3.13 mg/mL) and moderate α-amylase inhibitory activity (IC50, >10 mg/mL). Additionally, ZME exhibited potent peroxyl radical scavenging linked antioxidant activity (0.53/TE 1 μM). The in vivo study using SD rat and db/db mice models also showed that ZME reduces postprandial increases of blood glucose level after an oral administration of sucrose by possibly acting as an intestinal α-glucosidase inhibitor (ZME 0.1 g/kg 55.61 ± 13.24 mg/dL) CONCLUSION: The results indicate that Zingiber mioga extracts exhibited significant in vitro α-glucosidase inhibition and antioxidant activity. Additionally, the tested extracts demonstrated in vivo anti-hyperglycemic effects using SD rat and db/db mice models. Our findings provide a strong rationale for the further evaluation of Zingiber mioga for the potential to contribute as a useful dietary strategy to manage postprandial hyperglycemia.
- Subjects :
- Animals
Blood Glucose drug effects
Diabetes Mellitus, Experimental prevention & control
Female
Glycoside Hydrolase Inhibitors therapeutic use
Mice
Mice, Inbred C57BL
Prediabetic State drug therapy
Rats
Rats, Sprague-Dawley
Sucrase antagonists & inhibitors
alpha-Glucosidases metabolism
Diabetes Mellitus, Type 2 prevention & control
Enzyme Inhibitors therapeutic use
Hyperglycemia drug therapy
Hypoglycemic Agents therapeutic use
Plant Extracts therapeutic use
Zingiberaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6882
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- BMC complementary and alternative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27036710
- Full Text :
- https://doi.org/10.1186/s12906-016-1090-4