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Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes.
- Source :
-
Scientific reports [Sci Rep] 2016 Mar 31; Vol. 6, pp. 23795. Date of Electronic Publication: 2016 Mar 31. - Publication Year :
- 2016
-
Abstract
- We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN(+) LLC. Some cortical NeuN(+) neurons, GFAP(+) glia limitans astrocytes, Iba-1(+) microglia and S100β(+) ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes.
- Subjects :
- Animals
Astrocytes metabolism
Autophagy
Beclin-1 genetics
Beclin-1 metabolism
Calcium-Binding Proteins genetics
Calcium-Binding Proteins metabolism
Cell Count
Cerebral Cortex metabolism
Cerebral Cortex ultrastructure
Female
Gene Expression
Glial Fibrillary Acidic Protein genetics
Glial Fibrillary Acidic Protein metabolism
Histocytochemistry
Lipid Droplets metabolism
Meninges metabolism
Meninges ultrastructure
Mice
Mice, Inbred BALB C
Microfilament Proteins genetics
Microfilament Proteins metabolism
Microglia metabolism
Microtubule-Associated Proteins genetics
Microtubule-Associated Proteins metabolism
Neurogenesis
Neurons metabolism
Perilipin-1 genetics
Perilipin-1 metabolism
Phenotype
S100 Calcium Binding Protein beta Subunit genetics
S100 Calcium Binding Protein beta Subunit metabolism
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
beta-Galactosidase genetics
beta-Galactosidase metabolism
Aging metabolism
Astrocytes ultrastructure
Lipid Droplets ultrastructure
Microglia ultrastructure
Neurons ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27029648
- Full Text :
- https://doi.org/10.1038/srep23795