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[SPECTRUM OF ANTIBIOTIC RESISTANCE AND PREVALENCE OF OXA-CARBAPENEMASES AMONG ACINETOBACTER BAUMANNII STRAINS, ISOLATED FROM PATIENTS OF SURGICAL AND REANIMATION DEPARTMENTS IN MOSCOW].
- Source :
-
Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2016 Jan-Feb (1), pp. 40-5. - Publication Year :
- 2016
-
Abstract
- Aim: Characterize spectrum of antibiotics resistance of Acinetobacter baumannii strains, isolated from patients of 8 surgical and reanimation departments of 3 medical institution of Moscow, and determine molecular-genetic mechanisms of stability of their carbapenem-resistant forms.<br />Materials and Methods: 95 strains of A. baumannii, isolated from patients of reanimation and surgical departments of Moscow in 2012-2014, were studied. Sensitivity of strains to antibiotics was tested phenotypically according to recommendations of EUCAST. The presence of VIM, IMP, OXA-23, OXA-40, OXA-48, OXA-58 and NDM genes in the studied strains was determined by polymerase chain reaction in real time.<br />Results: 86.3% of strains turnedout to be non-sensitive to carbapenems, sensitive--13.7%. 80.0% of strains were non-sensitive to gentamicin, 80.0% of strains--to netilmicin, 94.7% of strains--to ciprofloxacin 2.1%--to colistin. 91.6% of isolates have shown non-sensitivity to members of 2 and more classes of antibiotics, 78.9% of strains--to members of 3 classes. 2 strains were panresistant, 4.2% (4/95) of the isolates were sensitive to all the classes of antibiotics. Metallo-β-lactamases were not detected. Genes of carbapenemases (OXA-23 and/or OXA-40) were detected in 85.3% (81/95) of strains, characterized phenotypically as non-sensitive to carbapenems.<br />Conclusion: The results obtained shown an increase of resistance to carbapenems and multiple resistance in clinically significant strains of A. baumannii. Resistance to carbapenems is associated with OXA-23 and OXA-40 genes. The conclusions allow to justify perspectives of introduction of technologies of molecular-genetic testing of antibiotics resistance.
- Subjects :
- Acinetobacter Infections drug therapy
Acinetobacter Infections microbiology
Acinetobacter baumannii enzymology
Acinetobacter baumannii genetics
Acinetobacter baumannii isolation & purification
Adult
Bacterial Proteins
Child
Gene Expression
Humans
Intensive Care Units
Microbial Sensitivity Tests
Moscow epidemiology
Prevalence
Real-Time Polymerase Chain Reaction
Surgical Procedures, Operative
beta-Lactamases metabolism
Acinetobacter Infections epidemiology
Acinetobacter baumannii drug effects
Anti-Bacterial Agents pharmacology
Carbapenems pharmacology
Drug Resistance, Multiple, Bacterial genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0372-9311
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhurnal mikrobiologii, epidemiologii i immunobiologii
- Publication Type :
- Academic Journal
- Accession number :
- 27029115