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Huannao Yicong Decoction () extract reduces inflammation and cell apoptosis in Aβ 1-42 -induced Alzheimer's disease model of rats.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2017 Sep; Vol. 23 (9), pp. 672-680. Date of Electronic Publication: 2016 Mar 29. - Publication Year :
- 2017
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Abstract
- Objective: Huannao Yicong Decoction (, HYD), an effective herbal formula against Alzheimer's disease (AD), has been proven to have neuroprotective action in amyloid β-protein <subscript>1-42</subscript> (Aβ <subscript>1-42</subscript> )-induced rat model. This study was designed to characterize mechanisms by which HYD leads to suppression of inflflammation and apoptosis in the brains of Aβ <subscript>1-42</subscript> -induced rat.<br />Methods: A total of 72 rats were divided into 6 groups, which were referred to as: sham operation group, model group, donepezil-treated group, HYD low-dose group (HYDL), HYD middle-dose group (HYDM) and HYD high-dose group (HYDH). Rats in HYDL, HYDM and HYDH were injected with Aβ <subscript>1-42</subscript> at the CA1 region of hippocampus to form AD model and were fed the HYD extract at different dose of 3.78, 7.56 and 18.90 g crude drug/kg. The behavioral changes of rats were evaluated by Morris water maze (MWM) before sacrififice. Pathological changes of the brain tissue were evaluated using hematoxylin eosin (HE) staining. The levels of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) were measured by radioimmunoassay. The levels of Aβ and proteins that are associated with apoptosis such as B-cell lymphoma-2 protein (Bcl-2), Bcl-2-associated X protein (Bax), cysteine-aspartic protease (caspase)-3, -8, -9 and -12 in serum were measured by immunohistochemistry.<br />Results: Compared with the sham operation group, the spatial learning and memory abilities of AD rats were signifificantly decreased (P<0.05 or P<0.01; Expressions of IL-1, TNF-α, Aβ and apoptosis-signaling proteins caspase-3, -8, -9, -12 were signifificantly up-regulated (P<0.05 or P<0.01). The ratio of Bcl-2 to Bax were signifificantly decreased in the model group (P<0.01). When treated with HYD extract, the spatial learning and memory abilities of AD-model rats were significantly increased (P<0.05 or P<0.01), IL-1, TNF-α, Aβ, caspase-3, -8, -9 and -12 were down-regulated (P<0.05 or P<0.01), and the ratio of Bcl-2 to Bax were reduced (P<0.05 or P<0.01).<br />Conclusions: HYD extract can improve the learning and memory ability defificits, alleviate the inflflammatory response and pathological manifestations induced by Aβ <subscript>1-42</subscript> injection in the rat model of AD. HYD down-regulates the levels of IL-1, TNF-α and Aβ, and decreases the rate of apoptosis by modulating apoptosis-signaling-related proteins such as caspase-3, -8, -9, and -12.
- Subjects :
- Alzheimer Disease blood
Alzheimer Disease complications
Amyloid beta-Peptides
Animals
Caspases metabolism
Chromatography, High Pressure Liquid
Disease Models, Animal
Female
Hippocampus drug effects
Hippocampus pathology
Inflammation blood
Inflammation complications
Interleukin-1 blood
Interleukin-1 metabolism
Male
Peptide Fragments
Rats, Sprague-Dawley
Spatial Learning drug effects
Tumor Necrosis Factor-alpha blood
Tumor Necrosis Factor-alpha metabolism
bcl-2-Associated X Protein metabolism
Alzheimer Disease drug therapy
Alzheimer Disease pathology
Apoptosis drug effects
Drugs, Chinese Herbal therapeutic use
Inflammation drug therapy
Inflammation pathology
Plant Extracts therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1672-0415
- Volume :
- 23
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27022730
- Full Text :
- https://doi.org/10.1007/s11655-016-2255-1