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Profile and bioavailability analysis of myo-inositol phosphates in rye bread supplemented with phytases: a study using an in vitro method and Caco-2 monolayers.
- Source :
-
International journal of food sciences and nutrition [Int J Food Sci Nutr] 2016 Jun; Vol. 67 (4), pp. 454-60. Date of Electronic Publication: 2016 Mar 28. - Publication Year :
- 2016
-
Abstract
- Commercial preparations of 6-phytase A alone and in combination with phytase B were used in rye breadmaking. Determination of bioavailability of myo-inositol phosphates from bread was performed by an in vitro digestion method followed by the measurement of an uptake by Caco-2 cells in culture. In bread supplemented with a combination of 6-phytase A and phytase B, a significant reduction in phytate content was observed from 3.62 μmol/g in the control to 0.7 μmol/g. Bioavailability of phytate estimated by an in vitro method simulating digestion in the human alimentary tract was 9% in the bread supplemented with phytase B, 7% (6-phytase A) and 50% in the control bread. In cell culture, the bioaccessibilities of inositol triphosphates from bread baked with the addition of 6-phytase A was higher by 36% as compared to the samples baked with phytase B and by 32% in breads baked with combination of both phytases.
- Subjects :
- Anion Exchange Resins
Caco-2 Cells
Chromatography, High Pressure Liquid
Dietary Fiber analysis
Digestion
Flour analysis
Humans
Inositol 1,4,5-Trisphosphate analysis
Inositol 1,4,5-Trisphosphate metabolism
Inositol Phosphates analysis
Isoenzymes
Models, Biological
Nutritive Value
Phosphorylation
Phytic Acid analysis
Phytic Acid metabolism
Poland
6-Phytase metabolism
Bread analysis
Dietary Fiber metabolism
Food Additives metabolism
Inositol Phosphates metabolism
Intestinal Absorption
Secale chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1465-3478
- Volume :
- 67
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of food sciences and nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 27019314
- Full Text :
- https://doi.org/10.3109/09637486.2016.1162769