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Linker histone variant H1T targets rDNA repeats.
Linker histone variant H1T targets rDNA repeats.
- Source :
-
Epigenetics [Epigenetics] 2016 Apr 02; Vol. 11 (4), pp. 288-302. Date of Electronic Publication: 2016 Mar 28. - Publication Year :
- 2016
-
Abstract
- H1T is a linker histone H1 variant that is highly expressed at the primary spermatocyte stage through to the early spermatid stage of spermatogenesis. While the functions of the somatic types of H1 have been extensively investigated, the intracellular role of H1T is unclear. H1 variants specifically expressed in germ cells show low amino acid sequence homology to somatic H1s, which suggests that the functions or target loci of germ cell-specific H1T differ from those of somatic H1s. Here, we describe the target loci and function of H1T. H1T was expressed not only in the testis but also in tumor cell lines, mouse embryonic stem cells (mESCs), and some normal somatic cells. To elucidate the intracellular localization and target loci of H1T, fluorescent immunostaining and ChIP-seq were performed in tumor cells and mESCs. We found that H1T accumulated in nucleoli and predominantly targeted rDNA repeats, which differ from somatic H1 targets. Furthermore, by nuclease sensitivity assay and RT-qPCR, we showed that H1T repressed rDNA transcription by condensing chromatin structure. Imaging analysis indicated that H1T expression affected nucleolar formation. We concluded that H1T plays a role in rDNA transcription, by distinctively targeting rDNA repeats.
- Subjects :
- Amino Acid Sequence genetics
Animals
Cell Nucleolus genetics
Chromatin genetics
DNA, Ribosomal biosynthesis
Gene Expression Regulation, Developmental
Germ Cells metabolism
Histones biosynthesis
Male
Mice
Mouse Embryonic Stem Cells metabolism
Sequence Homology, Amino Acid
Spermatids growth & development
Spermatids metabolism
Spermatocytes growth & development
Spermatocytes metabolism
Testis growth & development
Testis metabolism
DNA, Ribosomal genetics
Histones genetics
Spermatogenesis genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1559-2308
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Epigenetics
- Publication Type :
- Academic Journal
- Accession number :
- 27018843
- Full Text :
- https://doi.org/10.1080/15592294.2016.1159369