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Isolation of a Novel Fusogenic Orthoreovirus from Eucampsipoda africana Bat Flies in South Africa.
- Source :
-
Viruses [Viruses] 2016 Feb 29; Vol. 8 (3), pp. 65. Date of Electronic Publication: 2016 Feb 29. - Publication Year :
- 2016
-
Abstract
- We report on the isolation of a novel fusogenic orthoreovirus from bat flies (Eucampsipoda africana) associated with Egyptian fruit bats (Rousettus aegyptiacus) collected in South Africa. Complete sequences of the ten dsRNA genome segments of the virus, tentatively named Mahlapitsi virus (MAHLV), were determined. Phylogenetic analysis places this virus into a distinct clade with Baboon orthoreovirus, Bush viper reovirus and the bat-associated Broome virus. All genome segments of MAHLV contain a 5' terminal sequence (5'-GGUCA) that is unique to all currently described viruses of the genus. The smallest genome segment is bicistronic encoding for a 14 kDa protein similar to p14 membrane fusion protein of Bush viper reovirus and an 18 kDa protein similar to p16 non-structural protein of Baboon orthoreovirus. This is the first report on isolation of an orthoreovirus from an arthropod host associated with bats, and phylogenetic and sequence data suggests that MAHLV constitutes a new species within the Orthoreovirus genus.
- Subjects :
- Animals
Chiroptera parasitology
Cluster Analysis
Microscopy, Electron, Transmission
Molecular Weight
Orthoreovirus ultrastructure
Phylogeny
Sequence Homology
Sequence Homology, Amino Acid
South Africa
Viral Proteins chemistry
Viral Proteins genetics
Virion ultrastructure
Diptera virology
Genome, Viral
Orthoreovirus classification
Orthoreovirus isolation & purification
RNA, Viral genetics
Sequence Analysis, DNA
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 27011199
- Full Text :
- https://doi.org/10.3390/v8030065