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Effect of Intratracheal Administration of Adipose-derived Stromal Cells on Bleomycin-induced Lung Injury in a Rat Model.
- Source :
-
Osaka city medical journal [Osaka City Med J] 2015 Dec; Vol. 61 (2), pp. 81-91. - Publication Year :
- 2015
-
Abstract
- Background: Mesenchymal stromal cells (MSCs) have been intensively investigated in regenerative medicine. Among the different types of MSCs, adipose tissue-derived stromal cells (ASCs) can be obtained with relatively less invasive techniques. Since ASC administration is a candidate strategy for the treatment of refractory diseases including pulmonary fibrosis, we investigated whether intratracheal injection of ASCs had therapeutic potential against bleomycin (BLM)-induced lung injury in rats.<br />Methods: BLM was intratracheally administered to rats, and 1 week later ASCs were harvested. Two weeks after BLM treatment, ASCs or phosphate-buffered saline (PBS) were injected autologously into the rats via the trachea A semi-quantitative histological evaluation was conducted to assess the injured lungs, followed by cell tracing at 3 or 6 weeks after BLM instillation.<br />Results: ASC administration did not affect the severity of lung damage on the third week after BLM exposure, but prevented further aggravation of the lung injury, as apparent on the sixth week. A fluorescent cell tracer revealed that the majority of ASCs did not appear to have penetrated inside the lung region injured by BLM on the third week after BLM instillation, but some of these cells sprouted deep into the thick distorted architecture of the injured lung on the sixth week after the BLM instillation.<br />Conclusions: The results of the present study suggest that ASCs may play a role in the prevention of ongoing aggravation of lung injury in the long term.
- Subjects :
- Animals
Antibiotics, Antineoplastic administration & dosage
Disease Models, Animal
Drug Administration Routes
Male
Rats
Rats, Wistar
Regenerative Medicine methods
Treatment Outcome
Bleomycin administration & dosage
Lung pathology
Lung Injury chemically induced
Lung Injury pathology
Lung Injury therapy
Mesenchymal Stem Cell Transplantation methods
Pulmonary Fibrosis therapy
Trachea
Subjects
Details
- Language :
- English
- ISSN :
- 0030-6096
- Volume :
- 61
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Osaka city medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 26995852