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Targeted expression of cystatin restores fertility in cysteine protease induced male sterile tobacco plants.
- Source :
-
Plant science : an international journal of experimental plant biology [Plant Sci] 2016 May; Vol. 246, pp. 52-61. Date of Electronic Publication: 2016 Feb 12. - Publication Year :
- 2016
-
Abstract
- Fertility restoration in male sterile plants is an essential requirement for their utilization in hybrid seed production. In an earlier investigation, we have demonstrated that the targeted expression of a cysteine protease in tapetal cell layer resulted in complete male sterility in tobacco transgenic plants. In the present investigation, we have used a cystatin gene, which encodes for a cysteine protease inhibitor, from a wild peanut, Arachis diogoi and developed a plant gene based restoration system for cysteine protease induced male sterile transgenic tobacco plants. We confirmed the interaction between the cysteine protease and a cystatin of the wild peanut, A. diogoi through in silico modeling and yeast two-hybrid assay. Pollen from primary transgenic tobacco plants expressing cystatin gene under the tapetum specific promoter- TA29 restored fertility on cysteine protease induced male sterile tobacco plants developed earlier. This has confirmed the in vivo interaction of cysteine protease and cystatin in the tapetal cells, and the inactivation of cysteine protease and modulation of its negative effects on pollen fertility. Both the cysteine protease and cystatin genes are of plant origin in contrast to the analogous barnase-barstar system that deploys genes of prokaryotic origin. Because of the deployment of genes of plant origin, this system might not face biosafety problems in developing hybrids in food crops.<br /> (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Arachis enzymology
Chromosome Segregation
Computer Simulation
Crosses, Genetic
Fertility
Models, Molecular
Plants, Genetically Modified
Pollen anatomy & histology
Pollen metabolism
Pollen ultrastructure
Protein Binding
Two-Hybrid System Techniques
Cystatins metabolism
Cysteine Proteases metabolism
Gene Targeting
Plant Infertility
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2259
- Volume :
- 246
- Database :
- MEDLINE
- Journal :
- Plant science : an international journal of experimental plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 26993235
- Full Text :
- https://doi.org/10.1016/j.plantsci.2016.02.010