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Renal endothelial protein C receptor expression and shedding during diabetic nephropathy.
- Source :
-
Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2016 Jun; Vol. 14 (6), pp. 1171-82. Date of Electronic Publication: 2016 May 06. - Publication Year :
- 2016
-
Abstract
- Unlabelled: Essentials Endothelial protein C receptor (EPCR) promotes diabetic nephropathy (DN) outcome improvement. Renal expression and shedding of EPCR were measured in diabetic patients with or without DN. Inhibition of metalloproteinase-driven EPCR shedding restored glomerular endothelium phenotype. EPCR shedding through metalloproteinase ADAM17 contributes to the worsening of DN.<br />Summary: Background Diabetic nephropathy (DN) represents the leading cause of end-stage renal disease. The endothelial protein C receptor (EPCR) and its ligand (activated protein C) have been shown to ameliorate the phenotype of DN in mice. EPCR activity can be regulated by proteolytic cleavage involving ADAMs, yielding a soluble form of EPCR (sEPCR). Objective To characterize the renal expression and shedding of EPCR during DN. Methods EPCR levels were measured in plasma, urine and biopsy samples of diabetic patients with (n = 73) or without (n = 63) DN. ADAM-induced cleavage of EPCR was investigated in vitro with a human glomerular endothelium cell line. Results DN patients showed higher plasma and urinary levels of sEPCR than diabetic controls (112.2 versus 135.2 ng mL(-1) and 94.35 versus 140.6 ng mL(-1) , respectively). Accordingly, glomerular endothelial EPCR expression was markedly reduced in patients with DN, and this was associated with increased glomerular expression of ADAM-17 and ADAM-10. In vitro, EPCR shedding was induced by incubation of glomerular endothelium in high-glucose medium, and this shedding was suppressed by ADAM-17 inhibition or silencing, which led to improved vascular endothelial cadherin (VE-cadherin) expression and reduced mRNA expression of transforming growth factor (TGF)-β. In addition, EPCR silencing led to minor effects on VE-cadherin but to a significant increase in TGF-β mRNA expression. Conclusion Inhibition of ADAM-driven glomerular EPCR shedding restored the endothelial phenotype of glomerular endothelium, whereas EPCR silencing led to enhanced expression of TGF-β, a marker of endothelial-mesenchymal transition. These findings demonstrate that EPCR shedding driven by ADAMs contributes to the worsening of DN.<br /> (© 2016 International Society on Thrombosis and Haemostasis.)
- Subjects :
- ADAM10 Protein metabolism
ADAM17 Protein metabolism
Aged
Amyloid Precursor Protein Secretases metabolism
Biopsy
Cell Line
Diabetes Mellitus blood
Diabetes Mellitus pathology
Diabetes Mellitus urine
Diabetes Mellitus, Type 2 metabolism
Diabetes Mellitus, Type 2 pathology
Diabetic Nephropathies pathology
Endothelium pathology
Female
Gene Silencing
Humans
Kidney Glomerulus metabolism
Ligands
Male
Membrane Proteins metabolism
Metalloproteases metabolism
Middle Aged
Phenotype
RNA, Small Interfering metabolism
Transforming Growth Factor beta metabolism
Diabetic Nephropathies metabolism
Endothelial Protein C Receptor metabolism
Kidney metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7836
- Volume :
- 14
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of thrombosis and haemostasis : JTH
- Publication Type :
- Academic Journal
- Accession number :
- 26990852
- Full Text :
- https://doi.org/10.1111/jth.13315