Back to Search
Start Over
Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study.
- Source :
-
Veterinary research [Vet Res] 2016 Mar 17; Vol. 47, pp. 45. Date of Electronic Publication: 2016 Mar 17. - Publication Year :
- 2016
-
Abstract
- Porcine reproductive and respiratory syndrome (PRRS) is caused by PRRS virus (PRRSV), which infects primarily the respiratory tract of pigs. Thus intranasal (IN) delivery of a potent vaccine-adjuvant formulation is promising. In this study, PRRS-MLV (VR2332) was coadministered ± an adjuvant Mycobacterium vaccae whole cell lysate or CpG ODN through intramuscular (IM) or IN route as a mist, and challenged with a heterologous PRRSV 1-4-4 IN at 42 days post-vaccination (dpv). At 14 and 26 dpv, vaccine viral RNA copies were one log greater in the plasma of PRRS-MLV IM compared to IN vaccinated pigs, and the infectious replicating vaccine virus was detected only in the IM group. In PRRS-MLV ± adjuvant IM vaccinated pigs, reduced viral RNA load and absence of the replicating challenged virus was observed at 7, 10 and 14 days post-challenge (dpc). At 14 dpc, in BAL fluid ≥ 5 log viral RNA copies were detected in all the pig groups, but the replicating challenged virus was undetectable only in IM groups. Immunologically, virus neutralizing antibody titers in the plasma of IM (but not IN) vaccine groups was ≥ 8 against the vaccine and challenged viruses. At 26 dpv, PRRS-MLV IM (without adjuvant) received pigs had significantly increased population of CD4 and CD8 T cells in PBMC. At 14 dpc, relatively increased population of IFN-γ(+) total lymphocytes, NK, CD4, CD8 and γδ T cells were observed in the MLV-IM group. In conclusion, PRRS-MLV IM vaccination induced the virus specific T cell response in pigs, but still it is required to improve its cross-protective efficacy.
- Subjects :
- Adjuvants, Immunologic administration & dosage
Animals
Antibodies, Viral blood
Cross Protection
Immunity, Heterologous
Oligodeoxyribonucleotides administration & dosage
Oligodeoxyribonucleotides therapeutic use
Porcine Reproductive and Respiratory Syndrome immunology
Porcine Reproductive and Respiratory Syndrome virology
Swine
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated immunology
Vaccines, Attenuated therapeutic use
Viral Vaccines administration & dosage
Viral Vaccines therapeutic use
Adjuvants, Immunologic therapeutic use
Administration, Intranasal veterinary
Injections, Intramuscular veterinary
Mycobacterium immunology
Porcine Reproductive and Respiratory Syndrome prevention & control
Porcine respiratory and reproductive syndrome virus immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1297-9716
- Volume :
- 47
- Database :
- MEDLINE
- Journal :
- Veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 26988085
- Full Text :
- https://doi.org/10.1186/s13567-016-0331-3