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Cytokinin production by Pseudomonas fluorescens G20-18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis.
- Source :
-
Scientific reports [Sci Rep] 2016 Mar 17; Vol. 6, pp. 23310. Date of Electronic Publication: 2016 Mar 17. - Publication Year :
- 2016
-
Abstract
- Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinin-deficient loss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for this biocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbe-based, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.
- Subjects :
- Chromatography, High Pressure Liquid
Cytokinins analysis
Cytokinins pharmacology
Plant Diseases microbiology
Plant Growth Regulators pharmacology
Plant Leaves microbiology
Pseudomonas syringae drug effects
Pseudomonas syringae pathogenicity
Salicylic Acid pharmacology
Tandem Mass Spectrometry
Arabidopsis microbiology
Cytokinins biosynthesis
Pseudomonas fluorescens metabolism
Pseudomonas syringae growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26984671
- Full Text :
- https://doi.org/10.1038/srep23310