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Developmental regulation of chicken surfactant protein A and its localization in lung.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2016 Aug; Vol. 61, pp. 80-7. Date of Electronic Publication: 2016 Mar 11. - Publication Year :
- 2016
-
Abstract
- Surfactant Protein A (SP-A) is a collagenous C-type lectin (collectin) that plays an important role in the early stage of the host immune response. In chicken, SP-A (cSP-A) is expressed as a 26 kDa glycosylated protein in the lung. Using immunohistochemistry, cSP-A protein was detected mainly in the lung lining fluid covering the parabronchial epithelia. Specific cSP-A producing epithelial cells, resembling mammalian type II cells, were identified in the parabronchi. Gene expression of cSP-A markedly increased from embryonic day 14 onwards until the time of hatch, comparable to the SP-A homologue chicken lung lectin, while mannan binding lectin and collectins CL-L1 and CL-K1 only showed slightly changed expression during development. cSP-A protein could be detected as early as ED 18 in lung tissue using Western blotting, and expression increased steadily until day 28 post-hatch. Our observations are a first step towards understanding the role of this protein in vivo.<br /> (Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Animals, Newborn
Avian Proteins genetics
Collectins genetics
Gene Expression Regulation, Developmental
Humans
Immunity, Innate
Lung pathology
Mammals
Mannose-Binding Lectin metabolism
Phylogeny
Pulmonary Surfactant-Associated Protein A genetics
Avian Proteins metabolism
Chickens immunology
Lung metabolism
Pulmonary Surfactant-Associated Protein A metabolism
Respiratory Mucosa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 61
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 26976230
- Full Text :
- https://doi.org/10.1016/j.dci.2016.03.010