Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants.

Authors :: Elshikh M
Ahmed S
Funston S
Dunlop P
McGaw M
Marchant R
Banat IM
Source :: Biotechnology letters [Biotechnol Lett] 2016 Jun; Vol. 38 (6), pp. 1015-9. Date of Electronic Publication: 2016 Mar 11.
Publication Year :: 2016
Abstract: Objectives: To develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.<br />Results: A standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.<br />Conclusion: This new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts.

Subjects :: Anti-Bacterial Agents pharmacology
Gentamicins pharmacology
Indicators and Reagents
Microbial Sensitivity Tests instrumentation
Reproducibility of Results
Streptococcus drug effects
Tetracycline pharmacology
Microbial Sensitivity Tests methods
Oxazines
Surface-Active Agents pharmacology
Xanthenes

Full Text Access

Tools