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Determination of partial amino acid sequence of lipoate acetyltransferase of rat pyruvate dehydrogenase complex.
- Source :
-
Protein sequences & data analysis [Protein Seq Data Anal] 1989 Dec; Vol. 2 (6), pp. 441-4. - Publication Year :
- 1989
-
Abstract
- The partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with Achromobacter protease I. The results showed the amino-terminal sequence of the mature enzyme to be (N) Ser-Leu-Pro-Pro-His-Gln-Lys-Val-Pro-Leu-Pro-Ser- Leu-Ser-Pro-Thr-Met-Gln-Ala-Gly-Thr-Ile-Ala-Arg-Trp-Glu-Lys. In addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established.
- Subjects :
- Alcaligenes enzymology
Amino Acid Sequence
Amino Acids analysis
Animals
Binding Sites
Cattle
Chromatography, High Pressure Liquid
Dihydrolipoyllysine-Residue Acetyltransferase
Humans
Molecular Sequence Data
Molecular Weight
Peptide Hydrolases metabolism
Rats
Sequence Homology, Nucleic Acid
Acetyltransferases analysis
Acetyltransferases metabolism
Pyruvate Dehydrogenase Complex
Subjects
Details
- Language :
- English
- ISSN :
- 0931-9506
- Volume :
- 2
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Protein sequences & data analysis
- Publication Type :
- Academic Journal
- Accession number :
- 2696958