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Favourable effects of Bacillus subtilis and Bacillus licheniformis on experimental periodontitis in rats.
- Source :
-
Archives of oral biology [Arch Oral Biol] 2016 Jun; Vol. 66, pp. 108-19. Date of Electronic Publication: 2016 Feb 26. - Publication Year :
- 2016
-
Abstract
- Objectives: The purposes of this study were to evaluate, in rats: i) the effects of Bacillus species on the development of experimental periodontitis (EP) via microtomographic, immunological and hematological assays (Experiment 1-E1); ii) the effects of Bacillus species as adjuncts to scaling and root planing (SRP) for the treatment of EP via histomorphometric and immunohistochemical analyses (Experiment 2-E2).<br />Methods: In E1, 24 rats were divided into groups C1 (control), PROB1, EP1 and EP-PROB1. In groups with EP, the mandibular first molar of each animal received a ligature for 14 days. In groups PROB1, animals received Bacillus species for 44 days, starting 30 days before EP induction in Group EP-PROB1. In E2, 24 rats were assigned to groups C2 (control), PROB2, EP-SRP2 and EP-SRP-PROB2. In groups with SRP, EP was induced as described in E1. The ligatures were removed after 14 days and SRP was performed. In groups PROB2, animals received Bacillus species for 15 days, starting after SRP in Group EP-SRP-PROB2.<br />Results: In E1, Group EP1 presented bone loss (BL) and eosinophil numbers greater than Group EP-PROB1 (P<0.05). In Group EP-PROB1, the receptor activator of nuclear factor-kB ligand (RANKL)/osteoprotegerin (OPG) ratio was similar to that of groups without EP. In E2, Group EP-SRP-PROB2 presented fewer TRAP-positive osteoclasts, lower immunolabeling pattern for a proinflammatory cytokine and decreased BL and attachment loss than Group EP-SRP2 (P<0.05).<br />Conclusions: Bacillus species supplementation provided a protective effect against BL and enhanced the effects of SRP in the treatment of EP in rats.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Alveolar Bone Loss diagnostic imaging
Alveolar Bone Loss microbiology
Alveolar Bone Loss pathology
Alveolar Bone Loss therapy
Animals
Dental Scaling methods
Immunohistochemistry
Male
Molar microbiology
Osteoclasts metabolism
Osteoclasts microbiology
Osteoclasts pathology
Osteoprotegerin metabolism
Periodontitis blood
Periodontitis pathology
Random Allocation
Rats
Rats, Wistar
Root Planing methods
X-Ray Microtomography methods
Bacillus licheniformis physiology
Bacillus subtilis physiology
Periodontitis microbiology
Periodontitis therapy
Probiotics pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1506
- Volume :
- 66
- Database :
- MEDLINE
- Journal :
- Archives of oral biology
- Publication Type :
- Academic Journal
- Accession number :
- 26945169
- Full Text :
- https://doi.org/10.1016/j.archoralbio.2016.02.014