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Preparation of a Total Membrane Fraction from 3T3-L1 Adipocytes.
- Source :
-
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2016 Mar 01; Vol. 2016 (3), pp. pdb.prot083675. Date of Electronic Publication: 2016 Mar 01. - Publication Year :
- 2016
-
Abstract
- The dynamic nature of insulin-sensitive glucose transporter isoform 4 (GLUT4) storage vesicles (GSVs) makes their characterization challenging. Fractionation techniques can facilitate isolation of GSVs from insulin-sensitive cells. In this protocol, we describe preparation of a total membrane fraction from 3T3-L1 adipocytes. The resulting pellet contains all membranes and allows for easier identification of membrane proteins, including the insulin-sensitive pool of GLUT4. A method for concentration of the soluble fraction is also included.<br /> (© 2016 Cold Spring Harbor Laboratory Press.)
Details
- Language :
- English
- ISSN :
- 1559-6095
- Volume :
- 2016
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cold Spring Harbor protocols
- Publication Type :
- Academic Journal
- Accession number :
- 26933243
- Full Text :
- https://doi.org/10.1101/pdb.prot083675