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Evaluation of Immunophenotypic and Molecular Biomarkers for Sézary Syndrome Using Standard Operating Procedures: A Multicenter Study of 59 Patients.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2016 Jul; Vol. 136 (7), pp. 1364-1372. Date of Electronic Publication: 2016 Feb 28. - Publication Year :
- 2016
-
Abstract
- Differentiation between Sézary syndrome and erythrodermic inflammatory dermatoses can be challenging, and a number of studies have attempted to identify characteristic immunophenotypic changes and molecular biomarkers in Sézary cells that could be useful as additional diagnostic criteria. In this European multicenter study, the sensitivity and specificity of these immunophenotypic and recently proposed but unconfirmed molecular biomarkers in Sézary syndrome were investigated. Peripheral blood CD4(+) T cells from 59 patients with Sézary syndrome and 19 patients with erythrodermic inflammatory dermatoses were analyzed for cell surface proteins by flow cytometry and for copy number alterations and differential gene expression using custom-made quantitative PCR plates. Experiments were performed in duplicate in two independent centers using standard operating procedures with almost identical results. Sézary cells showed MYC gain (40%) and MNT loss (66%); up-regulation of DNM3 (75%), TWIST1 (69%), EPHA4 (66%), and PLS3 (66%); and down-regulation of STAT4 (91%). Loss of CD26 (≥80% CD4(+) T cells) and/or CD7 (≥40% CD4(+) T cells) and combination of altered expression of STAT4, TWIST1, and DNM3 or PLS3 could distinguish, respectively, 83% and 98% of patients with Sézary syndrome from patients with erythrodermic inflammatory dermatoses with 100% specificity. These additional diagnostic panels will be useful adjuncts in the differential diagnosis of Sézary syndrome versus erythrodermic inflammatory dermatoses.<br /> (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Aged
Aged, 80 and over
CD4-Positive T-Lymphocytes cytology
Diagnosis, Differential
Europe
Female
Flow Cytometry
Gene Dosage
Gene Expression Profiling
Gene Expression Regulation
Humans
Inflammation
Male
Middle Aged
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Sezary Syndrome immunology
Skin Diseases diagnosis
Skin Diseases immunology
Biomarkers analysis
Immunophenotyping standards
Sezary Syndrome diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 136
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 26930587
- Full Text :
- https://doi.org/10.1016/j.jid.2016.01.038