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Alcoholic vs non-alcoholic fatty liver in rats: distinct differences in endocytosis and vesicle trafficking despite similar pathology.
- Source :
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BMC gastroenterology [BMC Gastroenterol] 2016 Feb 29; Vol. 16, pp. 27. Date of Electronic Publication: 2016 Feb 29. - Publication Year :
- 2016
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Abstract
- Background: Non-alcoholic and alcoholic fatty liver disease (NAFLD and AFLD, respectively) are major health problems, as patients with either condition can progress to hepatitis, fibrosis, and cirrhosis. Although histologically similar, key differences likely exist in these two models. For example, altered content of several vesicle trafficking proteins have been identified in AFLD, but their content in NAFLD is unknown. In this study, we compared select parameters in NAFLD and AFLD in a rat model.<br />Methods: We fed either Lieber- DeCarli liquid control or alcohol-containing (35 % as calories) diet (AFLD model) or lean or high-fat (12 or 60 % derived from fat, respectively) pellets (NAFLD model) for 8-10 weeks, n = 8 in each model. Serum, hepatocytes and liver tissue were analyzed. Liver injury markers were measured in serum, triglyceride content and endocytosis (binding and internalization of (125)I- asialoorosomucoid) was measured in isolated hepatocytes, and content of selected trafficking proteins (Rab3D, Rab7 and Rab18) were determined in whole liver tissue.<br />Results: Although liver injury markers and triglyceride content were similar in both models, binding and internalization of (125)I- asialoorosomucoid was significantly impaired in the hepatocytes from AFLD, but not NAFLD, animals. In addition, protein content of the asialoglycoprotein receptor (ASGPR) and three trafficking proteins, Rab3D, Rab7and Rab18, were significantly decreased after alcohol, but not high-fat feeding. Levels of protein carbonylation, amount of glutathione stores, and lipid peroxidation were similar irrespective of the insult to the livers that resulted in fatty liver.<br />Conclusion: Impairments in protein trafficking in AFLD are likely a direct result of alcohol administration, and not a function of fatty liver.
- Subjects :
- Alanine Transaminase metabolism
Alkaline Phosphatase metabolism
Animals
Asialoglycoprotein Receptor genetics
Asialoglycoprotein Receptor metabolism
Bile Acids and Salts metabolism
Blotting, Western
Cholesterol metabolism
Diet, High-Fat
Disease Models, Animal
Ethanol toxicity
Fatty Liver, Alcoholic etiology
Immunohistochemistry
Membrane Proteins metabolism
Perilipin-2
Rats
Serum Albumin metabolism
Solvents toxicity
Triglycerides metabolism
rab GTP-Binding Proteins genetics
rab GTP-Binding Proteins metabolism
rab3 GTP-Binding Proteins genetics
rab3 GTP-Binding Proteins metabolism
rab7 GTP-Binding Proteins
Endocytosis physiology
Fatty Liver, Alcoholic metabolism
Hepatocytes metabolism
Non-alcoholic Fatty Liver Disease metabolism
RNA, Messenger metabolism
Transport Vesicles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1471-230X
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- BMC gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 26924554
- Full Text :
- https://doi.org/10.1186/s12876-016-0433-4