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Antagonistic activities of some Bifidobacterium sp. strains isolated from resident infant gastrointestinal microbiota on Gram-negative enteric pathogens.
- Source :
-
Anaerobe [Anaerobe] 2016 Jun; Vol. 39, pp. 39-44. Date of Electronic Publication: 2016 Feb 26. - Publication Year :
- 2016
-
Abstract
- The gastrointestinal microbiota contributes to the consolidation of the anti-infectious barrier against enteric pathogens. The purpose of this study was to investigate the influence of Bifidobacterium sp. strains, recently isolated from infant gastrointestinal microbiota on the in vitro growth and virulence features expression of enteropathogenic bacterial strains. The antibacterial activity of twelve Bifidobacterium sp. strains isolated from human feces was examined in vitro against a wide range of Gram negative pathogenic strains isolated from 30 infant patients (3 days to 5 years old) with diarrhea. Both potential probiotic strains (Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium catenulatum, Bifidobacterium breve, Bifidobacterium ruminantium) and enteropathogenic strains (EPEC, EIEC, Klebsiella pneumoniae, Salmonella sp., Yersinia enterocolitica, Pseudomonas aeruginosa) were identified by MALDI-TOF and confirmed serologically when needed. The bactericidal activity, growth curve, adherence to the cellular HEp-2 substratum and production of soluble virulence factors have been assessed in the presence of different Bifidobacterium sp. cultures and fractions (whole culture and free-cell supernatants). Among the twelve Bifidobacterium sp. strains, the largest spectrum of antimicrobial activity against 9 of the 18 enteropathogenic strains was revealed for a B. breve strain recently isolated from infant intestinal feces. The whole culture and free-cell supernatant of B. breve culture decreased the multiplication rate, shortened the log phase and the total duration of the growth curve, with an earlier entrance in the decline phase and inhibited the adherence capacity to a cellular substratum and the swimming/swarming motility too. These results indicate the significant probiotic potential of the B. breve strain.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacterial Adhesion drug effects
Child, Preschool
Diarrhea microbiology
Diarrhea physiopathology
Feces microbiology
Female
Gastrointestinal Microbiome physiology
Humans
Infant
Infant, Newborn
Klebsiella pneumoniae drug effects
Klebsiella pneumoniae growth & development
Male
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa growth & development
Salmonella drug effects
Salmonella growth & development
Virulence Factors biosynthesis
Yersinia enterocolitica drug effects
Yersinia enterocolitica growth & development
Antibiosis
Bifidobacterium physiology
Lactobacillus physiology
Probiotics pharmacology
Virulence Factors toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8274
- Volume :
- 39
- Database :
- MEDLINE
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 26921694
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2016.02.010