Back to Search
Start Over
Packaging of Campylobacter jejuni into Multilamellar Bodies by the Ciliate Tetrahymena pyriformis.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 2016 Apr 18; Vol. 82 (9), pp. 2783-90. Date of Electronic Publication: 2016 Apr 18 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Campylobacter jejuniis the leading cause of bacterial gastroenteritis worldwide. Transmission to humans occurs through consumption of contaminated food or water. The conditions affecting the persistence of C. jejuniin the environment are poorly understood. Some protozoa package and excrete bacteria into multilamellar bodies (MLBs). Packaged bacteria are protected from deleterious conditions, which increases their survival. We hypothesized that C. jejuni could be packaged under aerobic conditions by the amoeba Acanthamoeba castellanii or the ciliate Tetrahymena pyriformis, both of which are able to package other pathogenic bacteria.A. castellanii did not produce MLBs containing C. jejuni In contrast, when incubated with T. pyriformis,C. jejuni was ingested, packaged in MLBs, and then expelled into the milieu. The viability of the bacteria inside MLBs was confirmed by microscopic analyses. The kinetics of C. jejuni culturability showed that packaging increased the survival of C. jejuniup to 60 h, in contrast to the strong survival defect seen in ciliate-free culture. This study suggests that T. pyriformis may increase the risk of persistence of C. jejuniin the environment and its possible transmission between different reservoirs in food and potable water through packaging.<br /> (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Acanthamoeba castellanii growth & development
Acanthamoeba castellanii microbiology
Acanthamoeba castellanii ultrastructure
Aerobiosis
Animals
Campylobacter jejuni ultrastructure
Disease Vectors
Food Microbiology
Microbial Interactions
Microbial Viability
Microscopy, Electron, Transmission
Tetrahymena pyriformis ultrastructure
Water Microbiology
Water Supply
Campylobacter Infections transmission
Campylobacter jejuni physiology
Tetrahymena pyriformis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5336
- Volume :
- 82
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26921427
- Full Text :
- https://doi.org/10.1128/AEM.03921-15