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Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid.
- Source :
-
Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2016 Feb; Vol. 797, pp. 1-8. Date of Electronic Publication: 2015 Dec 18. - Publication Year :
- 2016
-
Abstract
- Diabetes mellitus (DM) is characterized by high blood glucose. Excessive production of free radicals may cause oxidative damage to DNA and other molecules, leading to complications of the disease. It may be possible to delay or reduce such damage by administration of antioxidants such as folic acid (FA). The objective of this study was to determine the effect of FA on nuclear abnormalities (NAs) in the oral mucosa of patients with DM. NAs (micronucleated cells, binucleated cells, pyknotic nuclei, karyorrhexis, karyolysis, abnormally condensed chromatin, and nuclear buds) were analyzed in 2000 cells from 45 healthy individuals (control group) and 55 patients with controlled or uncontrolled type I or II DM; 35 patients in the latter group were treated with FA. Samples were taken from the FA group before and after treatment. An increased rate of NAs was found in patients with DM in comparison with that of the control group (P<0.001). FA supplementation in patients with DM reduced the frequency of NAs (20.4 ± 8.0 before treatment vs. 10.5 ± 5.2 after treatment; P<0.001). The type I and type II DM and controlled and uncontrolled DM subgroups were analyzed in terms of sex, age, and smoking habit. The significantly reduced frequencies of buccal mucosa cells with micronuclei, binucleation, pyknosis, karyorrhexis, karyorrhexis+abnormally condensed chromatin, karyolysis, and nuclear buds produced by FA supplementation in DM patients (P<0.02) are consistent with the idea that free radicals are responsible for the increased frequency of NAs in DM patients.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Adult
Case-Control Studies
Cell Nucleus metabolism
Dietary Supplements
Female
Humans
Male
Middle Aged
Mouth Mucosa cytology
Young Adult
Cell Nucleus drug effects
Diabetes Mellitus, Type 1 drug therapy
Diabetes Mellitus, Type 2 drug therapy
Folic Acid therapeutic use
Micronuclei, Chromosome-Defective
Mouth Mucosa abnormalities
Mouth Mucosa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3592
- Volume :
- 797
- Database :
- MEDLINE
- Journal :
- Mutation research. Genetic toxicology and environmental mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 26921015
- Full Text :
- https://doi.org/10.1016/j.mrgentox.2015.12.003