[Semi-in vitro biosynthesis of cryptic lanthipeptide CLA 124 from Streptomyces clavuligerus].

Authors :: Zhang Z
Zhang L
Zhang J
Ma H
Sun S
Zhong J
Source :: Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2015 Nov 04; Vol. 55 (11), pp. 1402-8.
Publication Year :: 2015
Abstract: Objective: To obtain the cryptic lanthipeptide from Streptomyces clavuligerus by semi-in vitro biosynthesis that is a novel method for mining lanthipeptides resource from Streptomyces.<br />Methods: The core peptide of cryptic lanthipeptide was modified in E. coli by nisin modification system, and purified by affinity chromatography and High Performance Liquid Chromatography (HPLC). After the leader peptide was removed, the core peptide was obtained and its dehydration and cyclic structure were analyzed by MALDI-TOF MS and tandem MS.<br />Results: A novel lanthipeptide named CLA 124 with 4-fold dehydration 2 thioether bridges and one disulfide bridge was produced.<br />Conclusion: Cryptic lanthipeptides from Streptomyces could be produced by semi-in vitro biosynthesis.

Subjects :: Amino Acid Sequence
Bacteriocins chemistry
Bacteriocins genetics
Bacteriocins isolation & purification
Chromatography, High Pressure Liquid
Mass Spectrometry
Molecular Sequence Data
Peptides chemistry
Peptides genetics
Peptides isolation & purification
Streptomyces chemistry
Streptomyces genetics
Bacteriocins biosynthesis
Peptides metabolism
Streptomyces metabolism

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