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Immunoexpression of HPV 16/18 E6 and E7 oncoproteins in high-grade cervical squamous intraepithelial lesions in HIV-positive women.
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2016 Feb 19; Vol. 15 (1). Date of Electronic Publication: 2016 Feb 19. - Publication Year :
- 2016
-
Abstract
- The aim of this study was to assess the immunoexpression of human papillomavirus genotypes 16 and 18 (E6 and E7) oncoproteins in cervical high-grade squamous intraepithelial lesions (HSIL) of human immunodeficiency virus (HIV)-positive women. These results were also compared to the persistence and/or recurrence of lesions after loop electrosurgical excision procedure. Cervical samples from 158 patients were divided into three groups according to the presence or absence of HSIL in women who were or were not HIV-positive. By using the tissue microarray technique, immunohistochemistry was performed to analyze the expression of HPV 16/18 E6 and E7 oncoproteins. Cervical samples from 95 HIV-positive women and 63 HIV-negative women were studied. A statistically significant difference was found in the immunoexpression of E6 and E7 oncoproteins in samples from HIV-positive women with HSIL and that of women with non-neoplastic tissue (P < 0.001). There was also a statistically significant correlation between the immunoexpression of E6 (P = 0.012) and E7 (P < 0.001) oncoproteins in lesion persistence among HIV-positive women. Within the limitations of this study, the immunoexpression of HPV 16/18 E6 and E7 oncoproteins may have prognostic value regarding lesion persistence in HIV-positive women.
- Subjects :
- Adolescent
Adult
Aged
Coinfection
Female
HIV growth & development
HIV Infections genetics
HIV Infections immunology
HIV Infections virology
Human papillomavirus 16 genetics
Human papillomavirus 16 growth & development
Human papillomavirus 18 genetics
Human papillomavirus 18 growth & development
Humans
Immunohistochemistry
Middle Aged
Neoplasm Grading
Oncogene Proteins, Viral biosynthesis
Papillomavirus E7 Proteins biosynthesis
Papillomavirus Infections genetics
Papillomavirus Infections immunology
Papillomavirus Infections virology
Repressor Proteins biosynthesis
Squamous Intraepithelial Lesions of the Cervix genetics
Squamous Intraepithelial Lesions of the Cervix immunology
Squamous Intraepithelial Lesions of the Cervix virology
Tissue Array Analysis
Uterine Cervical Neoplasms genetics
Uterine Cervical Neoplasms immunology
Uterine Cervical Neoplasms virology
Gene Expression Regulation, Viral
HIV Infections pathology
Oncogene Proteins, Viral genetics
Papillomavirus E7 Proteins genetics
Papillomavirus Infections pathology
Repressor Proteins genetics
Squamous Intraepithelial Lesions of the Cervix pathology
Uterine Cervical Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 26909984
- Full Text :
- https://doi.org/10.4238/gmr.15017220