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Transcriptional identification and characterization of differentially expressed genes associated with embryogenesis in radish (Raphanus sativus L.).
- Source :
-
Scientific reports [Sci Rep] 2016 Feb 23; Vol. 6, pp. 21652. Date of Electronic Publication: 2016 Feb 23. - Publication Year :
- 2016
-
Abstract
- Embryogenesis is an important component in the life cycle of most plant species. Due to the difficulty in embryo isolation, the global gene expression involved in plant embryogenesis, especially the early events following fertilization are largely unknown in radish. In this study, three cDNA libraries from ovules of radish before and after fertilization were sequenced using the Digital Gene Expression (DGE) tag profiling strategy. A total of 5,777 differentially expressed transcripts were detected based on pairwise comparison in the three libraries (0_DAP, 7_DAP and 15_DAP). Results from Gene Ontology (GO) and pathway enrichment analysis revealed that these differentially expressed genes (DEGs) were implicated in numerous life processes including embryo development and phytohormones biosynthesis. Notably, some genes encoding auxin response factor (ARF ), Leafy cotyledon1 (LEC1) and somatic embryogenesis receptor-like kinase (SERK ) known to be involved in radish embryogenesis were differentially expressed. The expression patterns of 30 genes including LEC1-2, AGL9, LRR, PKL and ARF8-1 were validated by qRT-PCR. Furthermore, the cooperation between miRNA and mRNA may play a pivotal role in the radish embryogenesis process. This is the first report on identification of DEGs profiles related to radish embryogenesis and seed development. These results could facilitate further dissection of the molecular mechanisms underlying embryogenesis and seed development in radish.
- Subjects :
- CCAAT-Enhancer-Binding Proteins genetics
CCAAT-Enhancer-Binding Proteins metabolism
Gene Expression Profiling
Gene Library
Gene Ontology
MicroRNAs genetics
MicroRNAs metabolism
Molecular Sequence Annotation
Ovule embryology
Ovule metabolism
Plant Growth Regulators genetics
Plant Growth Regulators metabolism
Plant Proteins metabolism
Protein Kinases genetics
Protein Kinases metabolism
Raphanus embryology
Raphanus metabolism
Seeds growth & development
Seeds metabolism
Transcription Factors genetics
Transcription Factors metabolism
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Plant Proteins genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Raphanus genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26902837
- Full Text :
- https://doi.org/10.1038/srep21652