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Identification of phosphate groups important to self-splicing of the Tetrahymena rRNA intron as determined by phosphorothioate substitution.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1989 Dec 25; Vol. 17 (24), pp. 10281-93. - Publication Year :
- 1989
-
Abstract
- The group I intron from the rRNA precursor of Tetrahymena undergoes self-splicing. The intron RNA catalyst contains about 400 phosphate groups. Their role in catalysis has been investigated using phosphorothioate substituted RNA. In such RNA one of the peripheral oxygens of the phosphodiesters is replaced with sulfur. Incorporation of adenosine 5' phosphorothioate in either the 5' or 3' half of the ribozyme blocked splicing whereas incorporation of uridine 5' phosphorothioate only blocked splicing if the substitution was in the 3' half of the molecule. Modification-interference assays located two major and three minor inhibitory phosphorothioate substitutions suggesting that the corresponding phosphates play a significant role in self-splicing. These are all located in the most highly conserved region of the intron.
- Subjects :
- Adenosine Monophosphate analogs & derivatives
Adenosine Monophosphate metabolism
Animals
Base Sequence
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Catalytic
Thionucleotides metabolism
Introns
Organothiophosphorus Compounds metabolism
Phosphates metabolism
RNA Precursors genetics
RNA Splicing physiology
RNA, Ribosomal genetics
RNA, Ribosomal metabolism
Tetrahymena genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 17
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 2690016
- Full Text :
- https://doi.org/10.1093/nar/17.24.10281