Back to Search
Start Over
Novel approach for the ammonium removal by simultaneous heterotrophic nitrification and denitrification using a novel bacterial species co-culture.
- Source :
-
World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2016 Mar; Vol. 32 (3), pp. 36. Date of Electronic Publication: 2016 Feb 11. - Publication Year :
- 2016
-
Abstract
- Agricultural activities lead excessive emission of ammonia nitrogen in the environment and can profoundly interfere the equilibrium of the natural ecosystems leading to their contamination. Actually, the biological purification of wastewaters is the most adopted technique thanks to its several advantages such as high performance and low energy consumption. For this reason, two novel strains of Alcaligenes sp. S84S3 and Proteus sp. S19 genus were isolated from an activated sludge and applied in the treatment of ammonium and nitrite in aqueous solution. Under the optimum operating conditions of temperature (30 °C), pH (7), carbon substrate (2 g/L of glucose) and duration of incubation time (69 h), the strain Alcaligenes sp. S84S3 could oxidize 65% of the ammonium as high as 272.72 mg-NH4(+)/L. Moreover, during 48 h, the nitrate reduction rate performed by the strain Proteus S19 was about 99 % without production of nitrite intermediate (negligible concentration). Moreover, the coculture of the strains Alcaligenes sp. S84S3 and Proteus sp. S19 could eliminate 65.83% of the ammonium ions without production of toxic forms of nitrogen oxides during a short time of incubation (118 h) at the same operational conditions with providing the aeration in the first treatment phase. The coculture of our isolated strains is assumed to have a good potential for nitrification and denitrification reactions applied in the treatment of wastewater containing ammonium, nitrite and nitrate. As a result, we can consider that the mixed culture is a practical method in the treatment of high-strength ammonium wastewater with reducing of sludge production.
- Subjects :
- Alcaligenes growth & development
Alcaligenes isolation & purification
Carbon metabolism
Coculture Techniques
Hydrogen-Ion Concentration
Molecular Sequence Data
Proteus growth & development
Proteus isolation & purification
Sequence Analysis, DNA
Temperature
Time Factors
Water Pollutants metabolism
Alcaligenes metabolism
Ammonium Compounds metabolism
Denitrification
Nitrification
Nitrites metabolism
Proteus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0972
- Volume :
- 32
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- World journal of microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 26867597
- Full Text :
- https://doi.org/10.1007/s11274-015-2007-y