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Histological and transcriptome analyses of testes from Duroc and Meishan boars.
- Source :
-
Scientific reports [Sci Rep] 2016 Feb 11; Vol. 6, pp. 20758. Date of Electronic Publication: 2016 Feb 11. - Publication Year :
- 2016
-
Abstract
- Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR, and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars.
- Subjects :
- Animals
Breeding
Eosine Yellowish-(YS)
Gene Expression Profiling
Gene Expression Regulation, Developmental
Hematoxylin
Male
Neurotrophin 3 genetics
Neurotrophin 3 metabolism
Organ Size
Polymorphism, Single Nucleotide
Seminiferous Tubules ultrastructure
Sequence Analysis, RNA
Sertoli Cells ultrastructure
Species Specificity
Sperm Count
Spermatozoa ultrastructure
Swine
Seminiferous Tubules metabolism
Sertoli Cells metabolism
Sexual Maturation genetics
Spermatogenesis genetics
Spermatozoa metabolism
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26865000
- Full Text :
- https://doi.org/10.1038/srep20758