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Chemotyping the distribution of vitamin D metabolites in human serum.
- Source :
-
Scientific reports [Sci Rep] 2016 Feb 11; Vol. 6, pp. 21080. Date of Electronic Publication: 2016 Feb 11. - Publication Year :
- 2016
-
Abstract
- Most studies examining the relationships between vitamin D and disease or health focus on the main 25-hydroxyvitamin D3 (25(OH)D3) metabolite, thus potentially overlooking contributions and dynamic effects of other vitamin D metabolites, the crucial roles of several of which have been previously demonstrated. The ideal assay would determine all relevant high and low-abundant vitamin D species simultaneously. We describe a sensitive quantitative assay for determining the chemotypes of vitamin D metabolites from serum after derivatisation and ultra-high performance liquid chromatography-electrospray ionisation-tandem mass spectrometry (UHPLC-ESI-MS/MS). We performed a validation according to the 'FDA Guidance for Industry Bioanalytical Method Validation'. The proof-of-concept of the method was then demonstrated by following the metabolite concentrations in patients with chronic liver diseases (CLD) during the course of a vitamin D supplementation study. The new quantitative profiling assay provided highly sensitive, precise and accurate chemotypes of the vitamin D metabolic process rather than the usually determined 25(OH)D3 concentrations.
- Subjects :
- Calcitriol administration & dosage
Chromatography, High Pressure Liquid methods
Dietary Supplements
Humans
Liver metabolism
Liver pathology
Liver Cirrhosis diet therapy
Liver Cirrhosis pathology
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Vitamin D Deficiency diet therapy
Vitamin D Deficiency pathology
24,25-Dihydroxyvitamin D 3 blood
Calcifediol blood
Calcitriol blood
Liver Cirrhosis blood
Vitamin D Deficiency blood
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26864540
- Full Text :
- https://doi.org/10.1038/srep21080