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Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix.
- Source :
-
Experimental cell research [Exp Cell Res] 2016 Feb 15; Vol. 341 (2), pp. 207-17. Date of Electronic Publication: 2016 Feb 08. - Publication Year :
- 2016
-
Abstract
- Human hepatocytes are extensively needed in drug discovery and development. Stem cell-derived hepatocytes are expected to be an improved and continuous model of human liver to study drug candidates. Generation of endoderm-derived hepatocytes from human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, is a complex, challenging process requiring specific signals from soluble factors and insoluble matrices at each developmental stage. In this study, we used human liver progenitor HepaRG-derived acellular matrix (ACM) as a hepatic progenitor-specific matrix to induce hepatic commitment of hPSC-derived definitive endoderm (DE) cells. The DE cells showed much better attachment to the HepaRG ACM than other matrices tested and then differentiated towards hepatic cells, which expressed hepatocyte-specific makers. We demonstrate that Matrigel overlay induced hepatocyte phenotype and inhibited biliary epithelial differentiation in two hPSC lines studied. In conclusion, our study demonstrates that the HepaRG ACM, a hepatic progenitor-specific matrix, plays an important role in the hepatic differentiation of hPSCs.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1090-2422
- Volume :
- 341
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 26854693
- Full Text :
- https://doi.org/10.1016/j.yexcr.2016.02.006