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Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2016 Mar 04; Vol. 1436, pp. 100-8. Date of Electronic Publication: 2016 Jan 30. - Publication Year :
- 2016
-
Abstract
- A simple approach was developed for rapid preparation of polymeric monolithic columns in UV-transparent fused-silica capillaries via photoinitiated thiol-acrylate polymerization of pentaerythritol diacrylate monostearate (PEDAS) and trimethylolpropane tris(3-mercaptopropionate) (TPTM) within 10min, in which the acrylate homopolymerized and copolymerized with the thiol simultaneously. The morphology, permeability and chromatographic performance of the resulting poly(PEDAS-co-TPTM) monoliths were studied. It could be observed from SEM that the morphology of poly(PEDAS-co-TPTM) monolith was rather different from that of poly(PEDAS) monolith, which was fabricated via photo-induced free radical polymerization using PEDAS as the sole monomer. Compared with poly(PEDAS) monolith, poly(PEDAS-co-TPTM) monolith possessed better permeability when they were fabricated under the same preparation conditions. By adjusting the composition of porogenic solvents, poly(PEDAS-co-TPTM) monolith exhibited lower plate heights (15.7-17.7μm) than poly(PEDAS) monolith (19.1-37.9μm) in μLC. In addition, 66 unique peptides were positively identified on poly(PEDAS-co-TPTM) monolith when tryptic digest of four proteins was separated by μLC-MS/MS, demonstrating its potential in proteome analysis.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Chromatography, Liquid
Peptides analysis
Permeability
Photochemical Processes
Polymerization
Porosity
Proteins analysis
Tandem Mass Spectrometry
3-Mercaptopropionic Acid analogs & derivatives
3-Mercaptopropionic Acid chemistry
Acrylates chemistry
Propylene Glycols chemistry
Stearates chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1436
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 26852266
- Full Text :
- https://doi.org/10.1016/j.chroma.2016.01.063