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Angiotensin formation in the isolated rat hindlimb.
- Source :
-
Journal of hypertension [J Hypertens] 1989 Oct; Vol. 7 (10), pp. 789-98. - Publication Year :
- 1989
-
Abstract
- Local vascular generation of angiotensin was investigated in isolated perfused rat hindquarters. Extraction and combined high-performance liquid chromatography (HPLC)/radioimmunoassay analysis of hindlimb perfusate showed a spontaneous release of angiotensin I (Ang I; 5.0 +/- 3.4 fmol/h) and angiotensin II (Ang II; 31.8 +/- 7.9 fmol/h). Angiotensin converting enzyme (ACE) inhibition with captopril abolished Ang II release while Ang I levels increased more than 10-fold. Perfusion with purified hog renin caused a dose-dependent angiotensin release and vasoconstriction. The renin inhibitor H-142 abolished all effects of renin whereas ACE inhibition prevented Ang II formation and vasoconstriction but increased Ang I levels. Metabolism and pressor effects of synthetic tetradecapeptide renin substrate (TDP), Ang I and Ang II were studied using a recirculating rat hindlimb perfusion system. TDP-dependent formation of Ang I and II, and an increase in perfusion pressure was shown; ACE inhibition reduced but did not abolish Ang II formation and vasoconstriction. Ang I was converted to Ang II by about 50% during one pass through a hindlimb. This conversion was abolished by ACE inhibition. These data add support to the presence of a functional vascular renin-angiotensin system.
- Subjects :
- Angiotensin I isolation & purification
Angiotensin I metabolism
Angiotensin II isolation & purification
Angiotensin II metabolism
Angiotensin-Converting Enzyme Inhibitors
Animals
Captopril pharmacology
Chromatography, High Pressure Liquid
In Vitro Techniques
Male
Radioimmunoassay
Rats
Rats, Inbred Strains
Renin pharmacology
Vasoconstriction drug effects
Angiotensin I biosynthesis
Angiotensin II biosynthesis
Hindlimb metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0263-6352
- Volume :
- 7
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of hypertension
- Publication Type :
- Academic Journal
- Accession number :
- 2685116
- Full Text :
- https://doi.org/10.1097/00004872-198910000-00004