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The poly(A)-binding protein genes, EPAB, PABPC1, and PABPC3 are differentially expressed in infertile men with non-obstructive azoospermia.
- Source :
-
Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2016 Mar; Vol. 33 (3), pp. 335-348. Date of Electronic Publication: 2016 Feb 03. - Publication Year :
- 2016
-
Abstract
- Purpose: Azoospermia is one of the major causes of male infertility and is basically classified into obstructive (OA) and non-obstructive azoospermia (NOA). The molecular background of NOA still largely remains elusive. It has been shown that the poly(A)-binding proteins (PABPs) essentially play critical roles in stabilization and translational control of the mRNAs during spermatogenesis.<br />Methods: In the present study, we aim to evaluate expression levels of the PABP genes, EPAB, PABPC1, and PABPC3, in the testicular biopsy samples and in the isolated spermatocyte (SC) and round spermatid (RS) fractions obtained from men with various types of NOA including hypospermatogenesis (hyposperm), RS arrest, SC arrest, and Sertoli cell-only syndrome (SCO).<br />Results: In the testicular biopsy samples, both PABPC1 and PABPC3 mRNA expressions were gradually decreased from hyposperm to SCO groups (P < 0.05), whereas there was no remarkable difference for the EPAB expression among groups. The expression levels of cytoplasmically localized PABPC1 and PABPC3 proteins dramatically reduced from hyposperm to SCO groups (P < 0.05). In the isolated SC and RS fractions, the EPAB, PABPC1, and PABPC3 mRNA expressions were gradually decreased from hyposperm to SC arrest groups (P < 0.05). Similarly, both PABPC1 and PABPC3 proteins were expressed at higher levels in the SC and RS fractions from hyposperm group when compared to the SC and RS fractions from either RS arrest or SC arrest group (P < 0.05).<br />Conclusion: Our findings suggest that observed significant alterations in the PABPs expression may have an implication for development of different NOA forms.
- Subjects :
- Adult
Aged
Biopsy
Gene Expression Regulation
Humans
Male
Middle Aged
Oligospermia genetics
Oligospermia pathology
Poly(A)-Binding Protein I metabolism
Poly(A)-Binding Proteins metabolism
Sertoli Cell-Only Syndrome genetics
Sertoli Cell-Only Syndrome pathology
Testis physiopathology
Azoospermia genetics
Poly(A)-Binding Protein I genetics
Poly(A)-Binding Proteins genetics
Testis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-7330
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of assisted reproduction and genetics
- Publication Type :
- Academic Journal
- Accession number :
- 26843391
- Full Text :
- https://doi.org/10.1007/s10815-016-0654-z