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Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions.
- Source :
-
Nature communications [Nat Commun] 2016 Feb 02; Vol. 7, pp. 10363. Date of Electronic Publication: 2016 Feb 02. - Publication Year :
- 2016
-
Abstract
- Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, a primary site of leukocyte recruitment during inflammation. Here we show that endomucin abrogation on quiescent endothelial cells enables neutrophils to adhere firmly, via LFA-1-mediated binding to ICAM-1 constitutively expressed by endothelial cells. Moreover, TNF-α stimulation downregulates cell surface expression of endomucin concurrent with increased expression of adhesion molecules. Adenovirus-mediated expression of endomucin under inflammatory conditions prevents neutrophil adhesion in vitro and reduces the infiltration of CD45(+) and NIMP-R14(+) cells in vivo. These results indicate that endomucin prevents leukocyte contact with adhesion molecules in non-inflamed tissues and that downregulation of endomucin is critical to facilitate adhesion of leukocytes into inflamed tissues.
- Subjects :
- Aged
Animals
Female
Gene Expression Regulation physiology
Humans
Intercellular Adhesion Molecule-1 genetics
Intercellular Adhesion Molecule-1 metabolism
Lymphocyte Function-Associated Antigen-1 genetics
Lymphocyte Function-Associated Antigen-1 metabolism
Mice
Mice, Inbred C57BL
Neutrophils
RNA, Small Interfering
Sialomucins genetics
Skin cytology
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Cell Adhesion physiology
Endothelial Cells physiology
Inflammation metabolism
Leukocytes physiology
Sialomucins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 26831939
- Full Text :
- https://doi.org/10.1038/ncomms10363