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Influence of exercise training on proangiogenic TIE-2 monocytes and circulating angiogenic cells in patients with peripheral arterial disease.
- Source :
-
Clinical research in cardiology : official journal of the German Cardiac Society [Clin Res Cardiol] 2016 Aug; Vol. 105 (8), pp. 666-676. Date of Electronic Publication: 2016 Jan 30. - Publication Year :
- 2016
-
Abstract
- Background: Inflammation is the driving force in atherosclerosis. One central strategy in the treatment of peripheral arterial disease (PAD) is the promotion of angiogenesis. Here, proangiogenic Tie-2 expressing monocytes (TEM) and circulating angiogenic cells (CAC) play a crucial role. Exercise training (ET) is recommended in PAD patients at Fontaine stage II to promote angiogenesis.<br />Methods: 40 patients with intermittend claudication (IC) [2 groups: supervised ET (SET) vs. non-supervised ET (nSET), each n = 20] and 20 healthy controls were included in the study. Analysis of TEM and CAC was performed from whole blood by flow-cytometry. TEM were identified via CD45, CD86, CD14, CD16 and analysed for the expression of Tie-2. CAC were identified via their expression of CD45 (CD45dim), CD34 and VEGF-R2 (CD309/KDR). Follow up was performed after mean of 7.65 ± 1.62 months.<br />Results: In comparison to healthy controls, we found increased proportions of CAC (p < 0.0001) and similar TEM numbers in both ET groups. At follow-up (FU) TEM poroportions increased (p < 0.001) and CAC proportions decreased (p < 0.01), but both more significantly in SET (p < 0.001) than nSET (p = 0.01). Only in SET fibrinogen levels decreased and VEGF-A increased (both p < 0.05). Finally, we found in both ET groups a significant increase in absolute walking distance but with a higher individual increase in SET (p < 0.01). TEM and CAC proportions correlated inversely with the absolute walking distance (CAC: r = -0.296, p = 0.02; TEM: r = -0.270, p = 0.04) as well as with ABI (CAC: r = -0.394, p < 0.01; TEM: r = -0.382, p < 0.01).<br />Conclusions: ET influences the distribution of CAC and TEM proportions. nSET, although still effective in regard to an improved walking distance, is less effective in the influence of proangiogenic cells and inflammatory burden than SET. Our results indicate SET to be a more preferential exercise form, supporting the necessity to establish more SET programs.
- Subjects :
- Aged
Female
Humans
Inflammation Mediators blood
Intermittent Claudication blood
Intermittent Claudication diagnosis
Intermittent Claudication physiopathology
Lipids blood
Male
Middle Aged
Peripheral Arterial Disease blood
Peripheral Arterial Disease diagnosis
Peripheral Arterial Disease physiopathology
Pilot Projects
Recovery of Function
Time Factors
Treatment Outcome
Vascular Endothelial Growth Factor A blood
Biomarkers blood
Exercise Therapy
Exercise Tolerance
Intermittent Claudication therapy
Monocytes metabolism
Neovascularization, Physiologic
Peripheral Arterial Disease therapy
Receptor, TIE-2 blood
Subjects
Details
- Language :
- English
- ISSN :
- 1861-0692
- Volume :
- 105
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical research in cardiology : official journal of the German Cardiac Society
- Publication Type :
- Academic Journal
- Accession number :
- 26830098
- Full Text :
- https://doi.org/10.1007/s00392-016-0966-0