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Cth2 Protein Mediates Early Adaptation of Yeast Cells to Oxidative Stress Conditions.
- Source :
-
PloS one [PLoS One] 2016 Jan 29; Vol. 11 (1), pp. e0148204. Date of Electronic Publication: 2016 Jan 29 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in iron starvation conditions by promoting decay of the targeted molecules, in order to avoid excess iron consumption. This study shows that in the absence of Cth2 immediate upregulation of expression of several of the iron regulon genes (involved in high affinity iron uptake and intracellular iron redistribution) upon oxidative stress by hydroperoxide is more intense than in wild type conditions where Cth2 is present. The oxidative stress provokes a temporary increase in the levels of Cth2 (itself a member of the iron regulon). In such conditions Cth2 molecules accumulate at P bodies-like structures when the constitutive mRNA decay machinery is compromised. In addition, a null Δcth2 mutant shows defects, in comparison to CTH2 wild type cells, in exit from α factor-induced arrest at the G1 stage of the cell cycle when hydroperoxide treatment is applied. The cell cycle defects are rescued in conditions that compromise uptake of external iron into the cytosol. The observations support a role of Cth2 in modulating expression of diverse iron regulon genes, excluding those specifically involved in the reductive branch of the high-affinity transport. This would result in immediate adaptation of the yeast cells to an oxidative stress, by controlling uptake of oxidant-promoting iron cations.
- Subjects :
- G1 Phase Cell Cycle Checkpoints drug effects
G1 Phase Cell Cycle Checkpoints genetics
Gene Expression Profiling
Hydrogen Peroxide pharmacology
Ion Transport drug effects
Mating Factor
Oxidation-Reduction
Oxidative Stress
Peptides genetics
Peptides metabolism
RNA, Messenger metabolism
Regulon drug effects
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Signal Transduction
Time Factors
Tristetraprolin metabolism
Adaptation, Physiological genetics
Gene Expression Regulation, Fungal
Iron metabolism
RNA, Messenger genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Tristetraprolin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26824473
- Full Text :
- https://doi.org/10.1371/journal.pone.0148204