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Association between CTLA-4 rs231775 polymorphism and hepatocellular carcinoma susceptibility.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2015 Nov 01; Vol. 8 (11), pp. 15118-22. Date of Electronic Publication: 2015 Nov 01 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Aims: Our study aimed to investigate the association of cytotoxic T-lymphocyte antigen-4 (CTLA-4) rs231775 polymorphism with hepatocellular carcinoma (HCC) susceptibility.<br />Methods: Genotypes distribution of the control was tested by Hardy-Weinberg Equilibrium (HWE). CTLA-4 rs231775 polymorphism was analyzed in 80 patients with HCC and 78 healthy controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the expression level of CTLA-4 in the serum of all subjects was detected using enzyme linked immunosorbent assay (ELISA) kit. Odd ratio (OR) with 95% confidence interval (CI) were calculated by chi-squared test to determine the correlation of CTLA-4 rs231775 polymorphism and the risk of HCC.<br />Results: The genotypes frequencies of the control group were in accordance with HWE. The frequencies of genotype AA and allele A in CTLA-4 rs231775 polymorphism were significantly higher in cases than the control group (AA vs. GG: OR=2.81, P=0.043; A vs. G: OR=1.63, P=0.022). Meanwhile, the expression level of CTLA-4 was remarkably higher in cases compared with the controls. The association analysis indicated that AA genotype carriers exhibited highest level of CTLA-4 (P<0.01).<br />Conclusions: The genotype AA and allele A of CTLA-4 rs231775 polymorphism may have negative effects on HCC by modifying the expression and functions of CTLA-4.
- Subjects :
- Adult
Aged
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Female
Genotype
Humans
Male
Middle Aged
Polymorphism, Restriction Fragment Length
Carcinoma, Hepatocellular genetics
Genetic Predisposition to Disease genetics
Liver Neoplasms genetics
Membrane Transport Proteins genetics
Polymorphism, Single Nucleotide
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 8
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26823853