Back to Search
Start Over
Transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of Bordetella adenylate cyclase toxin.
- Source :
-
Pathogens and disease [Pathog Dis] 2016 Apr; Vol. 74 (3). Date of Electronic Publication: 2016 Jan 21. - Publication Year :
- 2016
-
Abstract
- Adenylate cyclase toxin-hemolysin (CyaA, ACT or AC-Hly) of the whooping cough agent Bordetella pertussis penetrates phagocytes expressing the integrin complement receptor 3 (CR3, CD11b/CD18, α(M)β(2) or Mac-1). CyaA translocates its adenylate cyclase (AC) enzyme domain into cell cytosol and catalyzes unregulated conversion of ATP to cAMP, thereby subverting cellular signaling. In parallel, CyaA forms small cation-selective membrane pores that permeabilize cells for potassium efflux, contributing to cytotoxicity of CyaA and eventually provoking colloid-osmotic cell lysis. To investigate whether the single-pass α-helical transmembrane segments of CR3 subunits CD11b and CD18 do directly participate in AC domain translocation and/or pore formation by the toxin, we expressed in CHO cells variants of CR3 that contained artificial transmembrane segments, or lacked the transmembrane segment(s) at all. The results demonstrate that the transmembrane segments of CR3 are not directly involved in the cytotoxic activities of CyaA but serve for maintaining CR3 in a conformation that is required for efficient toxin binding and action.<br /> (© FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Adenosine Triphosphate chemistry
Animals
Biological Transport physiology
CD11b Antigen genetics
CD18 Antigens genetics
CHO Cells
Cell Line
Cricetulus
Cyclic AMP biosynthesis
Humans
Macrophage-1 Antigen biosynthesis
Macrophage-1 Antigen genetics
Phagocytes metabolism
Signal Transduction physiology
Adenylate Cyclase Toxin metabolism
Bordetella pertussis metabolism
CD11b Antigen metabolism
CD18 Antigens metabolism
Macrophage-1 Antigen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2049-632X
- Volume :
- 74
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pathogens and disease
- Publication Type :
- Academic Journal
- Accession number :
- 26802078
- Full Text :
- https://doi.org/10.1093/femspd/ftw008