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Erwinia teleogrylli sp. nov., a Bacterial Isolate Associated with a Chinese Cricket.
- Source :
-
PloS one [PLoS One] 2016 Jan 22; Vol. 11 (1), pp. e0146596. Date of Electronic Publication: 2016 Jan 22 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- A bacterial isolate (SCU-B244T) was obtained in China from crickets (Teleogryllus occipitalis) living in cropland deserted for approximately 10 years. The isolated bacteria were Gram-negative, facultatively anaerobic, oxidase-negative rods. A preliminary analysis of the 16S rRNA gene sequence indicated that the strain belongs to either the genus Erwinia or Pantoea. Analysis of multilocus sequence typing based on concatenated partial atpD, gyrB and infB gene sequences and physiological and biochemical characteristics indicated that the strain belonged to the genus Erwinia, as member of a new species as it was distinct from other known Erwinia species. Further analysis of the 16S rRNA gene showed SCU-B244T to have 94.71% identity to the closest species of that genus, Erwinia oleae (DSM 23398T), which is below the threshold of 97% used to discriminate bacterial species. DNA-DNA hybridization results (5.78±2.52%) between SCU-B244T and Erwinia oleae (DSM 23398T) confirmed that SCU-B244T and Erwinia oleae (DSM 23398T) represent different species combined with average nucleotide identity values which range from 72.42% to 74.41. The DNA G+C content of SCU-B244T was 55.32 mol%, which also differs from that of Erwinia oleae (54.7 to 54.9 mol%). The polyphasic taxonomic approach used here confirmed that the strain belongs to the Erwinia group and represents a novel species. The name Erwinia teleogrylli sp. nov. is proposed for this novel taxon, for which the type strain is SCU-B244T (= CGMCC 1.12772T = DSM 28222T = KCTC 42022T).
- Subjects :
- Animals
Bacterial Typing Techniques
Base Composition genetics
China
Chlorpyrifos metabolism
DNA Gyrase genetics
DNA, Bacterial genetics
DNA, Ribosomal genetics
Erwinia classification
Erwinia genetics
Insecticides metabolism
Multilocus Sequence Typing
Prokaryotic Initiation Factor-2 genetics
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Transcription Factors genetics
Chlorpyrifos pharmacology
Drug Resistance genetics
Erwinia isolation & purification
Erwinia metabolism
Gryllidae drug effects
Gryllidae microbiology
Insecticides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26800121
- Full Text :
- https://doi.org/10.1371/journal.pone.0146596