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Cell culture density affects the proliferation activity of human adipose tissue stem cells.
- Source :
-
Cell biochemistry and function [Cell Biochem Funct] 2016 Jan; Vol. 34 (1), pp. 16-24. Date of Electronic Publication: 2016 Jan 17. - Publication Year :
- 2016
-
Abstract
- In this study, we investigated the effect of cell density on the proliferation activity of human mesenchymal stem cells (MSCs) derived from adipose tissue (AT-MSCs) over time in culture. Passage #4 (P4) and #12 (P12) AT-MSCs from two donors were plated at a density of 200 (culture condition 1, CC1) or 5000 (culture condition 2, CC2) cells cm(-2) . After 7 days of incubation, P4 and P12 AT-MSCs cultured in CC1 were thin and spindle-shaped, whereas those cultured in CC2 had extensive cell-to-cell contacts and an expanded cell volume. In addition, P4 and P12 AT-MSCs in CC1 divided more than three times, while those in CC2 divided less than once on average. Flow cytometric analysis using 5(6)-carboxyfluorescein diacetate N-succinimidyl ester dye showed that the fluorescence intensity of AT-MSCs was lower in CC1 than in CC2. Furthermore, expression of proliferation-associated genes, such as CDC45L, CDC20A and KIF20A, in P4 AT-MSCs was higher in CC1 than in CC2, and this difference was also observed in P12 AT-MSCs. These data demonstrated that cell culture density affects the proliferation activity of MSCs, suggesting that it is feasible to design a strategy to prepare suitable MSCs using specific culture conditions.<br /> (Copyright © 2016 John Wiley & Sons, Ltd.)
Details
- Language :
- English
- ISSN :
- 1099-0844
- Volume :
- 34
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell biochemistry and function
- Publication Type :
- Academic Journal
- Accession number :
- 26778408
- Full Text :
- https://doi.org/10.1002/cbf.3158