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Moderate intensity static magnetic fields affect mitotic spindles and increase the antitumor efficacy of 5-FU and Taxol.
- Source :
-
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2016 Jun; Vol. 109, pp. 31-40. Date of Electronic Publication: 2016 Jan 06. - Publication Year :
- 2016
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Abstract
- Microtubules are the fundamental components in mitotic spindle, which plays essential roles in cell division. It was well known that purified microtubules could be affected by static magnetic fields (SMFs) in vitro because of the diamagnetic anisotropy of tubulin. However, whether these effects lead to cell division defects was unknown. Here we find that 1T SMFs induce abnormal mitotic spindles and increase mitotic index. Synchronization experiments show that SMFs delay cell exit from mitosis and cause mitotic arrest. These mimic the cellular effects of a microtubule-targeting drug Paclitaxel (Taxol), which is frequently used in combination with 5-Fluorouracil (5-FU) and Cisplatin in cancer treatment. Using four different human cancer cell lines, HeLa, HCT116, CNE-2Z and MCF7, we find that SMFs increase the antitumor efficacy of 5-FU or 5-FU/Taxol, but not Cisplatin, which indicates that the SMF-induced combinational effects with chemodrugs are drug-specific. Our study not only reveals the effect of SMFs on microtubules to cause abnormal mitotic spindles and delay cells exit from mitosis, but also implies the potential applications of SMFs in combination with chemotherapy drugs 5-FU or 5-FU/Taxol, but not with Cisplatin in cancer treatment.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Antineoplastic Agents pharmacology
Cell Line, Tumor
Combined Modality Therapy methods
Fluorouracil pharmacology
Humans
Magnetic Fields
Neoplasms pathology
Paclitaxel pharmacology
Antineoplastic Agents therapeutic use
Fluorouracil therapeutic use
Magnetic Field Therapy methods
Mitosis
Neoplasms therapy
Paclitaxel therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1878-562X
- Volume :
- 109
- Database :
- MEDLINE
- Journal :
- Bioelectrochemistry (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 26775206
- Full Text :
- https://doi.org/10.1016/j.bioelechem.2016.01.001