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Peroxisome proliferator-activated receptor β/δ (PPARβ/δ) activates promyogenic signaling pathways, thereby promoting myoblast differentiation.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2016 Jan 29; Vol. 470 (1), pp. 157-162. Date of Electronic Publication: 2016 Jan 06. - Publication Year :
- 2016
-
Abstract
- Peroxisome proliferator-activated receptor β/δ (PPARβ/δ) regulates postnatal myogenesis by alleviating myostatin activity, but the molecular mechanisms by which it regulates myogenesis are not fully understood. In this study, we investigate molecular mechanisms of PPARβ/δ in myoblast differentiation. C2C12 myoblasts treated with a PPARβ/δ agonist, GW0742 exhibit enhanced myotube formation and muscle-specific gene expression. GW0742 treatment dramatically activates promyogenic kinases, p38MAPK and Akt, in a dose-dependent manner. GW0742-stimulated myoblast differentiation is mediated by p38MAPK and Akt, since it failed to restore myoblast differentiation repressed by inhibition of p38MAPK and Akt. In addition, GW0742 treatment enhances MyoD-reporter activities. Consistently, overexpression of PPARβ/δ enhances myoblast differentiation accompanied by elevated activation of p38MAPK and Akt. Collectively, these results suggest that PPARβ/δ enhances myoblast differentiation through activation of promyogenic signaling pathways.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Differentiation drug effects
Cell Differentiation physiology
Cell Line
Gene Expression Regulation, Developmental drug effects
Gene Expression Regulation, Developmental physiology
MAP Kinase Signaling System drug effects
MAP Kinase Signaling System physiology
Mice
Muscle Development drug effects
Muscle Development physiology
Muscle Fibers, Skeletal drug effects
PPAR gamma agonists
PPAR-beta agonists
Thiazoles administration & dosage
Muscle Fibers, Skeletal cytology
Muscle Fibers, Skeletal metabolism
Myoblasts cytology
Myoblasts metabolism
PPAR gamma metabolism
PPAR-beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 470
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 26768366
- Full Text :
- https://doi.org/10.1016/j.bbrc.2016.01.012