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False-negative type-specific glycoprotein G antibody responses in STI clinic patients with recurrent HSV-1 or HSV-2 DNA positive genital herpes, The Netherlands.
- Source :
-
Sexually transmitted infections [Sex Transm Infect] 2016 Jun; Vol. 92 (4), pp. 257-60. Date of Electronic Publication: 2016 Jan 11. - Publication Year :
- 2016
-
Abstract
- Objectives: Herpes simplex virus (HSV) type-discriminating antibody tests (glycoprotein G (gG) directed) are used to identify naïve persons and differentiate acute infections from recurrences. We studied test characteristics of three commercially available antibody tests in patients with recurrent (established by viral PCR tests) herpes simplex virus type 1 (HSV-1) or herpes simplex virus type 2 (HSV-2) genital herpes episodes.<br />Methods: Serum samples (at minimum 3 months after t=0) were examined for the presence of gG-1-specific or gG-2-specific antibodies using the HerpeSelect 1 and 2 Immunoblot IgG, the HerpeSelect 1 and 2 enzyme linked immunoassays IgG and the LIAISON HSV-1 and HSV-2 IgG indirect chemiluminescence immunoassays.<br />Results: The immunoblot was HSV-1 positive in 70.6% (95% CI 44.0% to 89.7%), the LIAISON in 88.2% (95% CI 63.5% to 98.5%) and the ELISA in 82.4% (95% CI 56.6% to 96.2%) of the 17 patients with a recurrent HSV-1 episode. From 33 patients with a recurrent HSV-2 episode, the immunoblot was HSV-2 positive in 84.8% (95% CI 68.1% to 94.9%), the LIAISON in 69.7% (95% CI 51.3% to 84.4%) and the ELISA in 84.8% (95% CI 68.1% to 94.9%). Among 15/17 (88.2%; 95% CI 63.5% to 98.5%) patients with HSV-1 and 30/33 (90.1%; 95% CI 75.7% to 98.1%) patients with HSV-2, HSV-1 or HSV-2 antibodies, respectively, were detected in at least one of the three antibody tests.<br />Conclusions: Commercial type-specific gG HSV-1 or HSV-2 antibody assays were false negative in 12-30% of patients with recurrent HSV-1 or HSV-2 DNA positive genital lesions. The clinical and epidemiological use of type-specific HSV serology can be hampered by false-negative results, especially if based on a single test.<br /> (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Subjects :
- Adult
Antibody Formation
Enzyme-Linked Immunosorbent Assay
False Negative Reactions
Female
Herpes Genitalis diagnosis
Herpes Genitalis epidemiology
Herpes Genitalis immunology
Herpesvirus 1, Human immunology
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human immunology
Herpesvirus 2, Human isolation & purification
Humans
Immunoblotting
Male
Middle Aged
Netherlands epidemiology
Predictive Value of Tests
Recurrence
Sexual Behavior
Antibodies, Viral immunology
Herpes Genitalis virology
Herpesvirus 1, Human genetics
Herpesvirus 2, Human genetics
Viral Envelope Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1472-3263
- Volume :
- 92
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Sexually transmitted infections
- Publication Type :
- Academic Journal
- Accession number :
- 26755775
- Full Text :
- https://doi.org/10.1136/sextrans-2015-052213